Evaluation of Ultraviolet-C on the Induction of Antibiotic Resistance in environmental Bacteria gamma resistance in Laboratory Conditions

Recently, bacterial infections have increased due to overuse of antibiotics and that has created problems. One of the most influential factors in bacteria is ultraviolet radiation, which can cause different behaviors and may eventually lead to mutation. In this study, ۳ bacteria of Bacillus cereus, Bacillus subtilis and micrococci that were sampled were exposed to ultraviolet light in two times of ۱۰ minutes and ۲۰ minutes. The antibiotic susceptibility of these three bacteria was assessed by measuring the inhibition zone diameter. The aim of this study was to evaluate ultraviolet radiation on the induction of antibiotic resistance in gamma-resistant environmental bacteria after exposure to ultraviolet radiation. Laboratory sampling was performed from a predetermined area in front of Gamma source. These three strains were exposed to ultraviolet light at two different times, cultured on Muller-Hinton agar and incubated at ۳۷ ° C. The inhibition zone diameter of these three bacteria was measured before and after UV-C irradiation. Finally, antibiotic susceptibility of these three species of bacteria was measured. The results of this study showed that ultraviolet radiation completely killed the test bacteria for ۲۰ minutes and test bacteria were reduced for ۱۰ minutes, compared to the control group. It also increased the resistance of Bacillus cereus to Azithromycin and Piperacillin and micrococci against AZM, PRL and Oxacillin This in turn leads to the spread and resistance to antibiotics. radiation resistance can indirectly transmit resistance to antibiotics and the strain can spread and expand the resistance due to its spread in the environment.

کلیدواژه ها:

micrococci ، Bacillus cereus ، Bacillus subtilis ، radiation