Isolation and Screening of Microbial Protease Enzymes from Soil

Proteases are enzymes that hydrolyze peptide bonds of proteins due to their highly selective modality and high activity at very low concentrations. Recently, microbial protease have been widely used in various industrial fields such as (cosmetics, food, pharmaceuticals, leather, detergents, livestock and poultry feed, etc.). By studying various sources, researchers have obtained protease enzymes from plants, animals, and microorganisms. two-thirds of the world's commercial proteases produced by microorganisms. One of the methods of production and development of protease enzymes from microbial sources is the use of microbial waste and isolation of microorganisms growing on them. The aim of this study was to screen and isolate proteolytic bacteria from the soil of slaughterhouse areas. Soil is an ideal source of many microorganisms that produce extracellular enzymes. soil samples were collected from the area around the slaughterhouse located in Charmshahr, Isfahan. Bacteria were isolated by dilution series technique and selected from ۱۰ −۸dilutions. Selective isolates were screened for extracellular protease production. For qualitative analysis (hydrolysis region), all isolates were inoculated in gelatin agar and skim milk plates and incubated in ۳۷𝐶۰. ۲ isolates (D۳ and G۲) with the largest clear area were selected. By the growth optimization technique and drawing the standard diagram, the best growth temperature and time were obtained at ۳۷ 𝐶۰ and ۲۰ hours, respectively .Isolates after Gram stain test for more accurate, identified by ۱۶S-rDNA ,The isolates were from the Bacillus family. By complementary techniques such as biochemical tests and by providing various protein sources such as blood and bone waste, selective isolates can be evaluated and used on an industrial scale.

کلیدواژه ها:

Microorganism ، waste ، protein ، proteolytic