Evaluation of the effect of Helicobacter pylori virulence factors on macrophage cells Times

Helicobacter pylori bacilli Gram-negative is one of the prominent features of this bacterium important pathogens such as urease, flagell, CagA, VacA, BabA, SabA, AlpA / AlpB, IceA, DupA, LPS and OipA. In addition, macrophages can and do initiate a central mediator between the innate and acquired immune systems, and the immune response to Helicobacter pylori is predominantly by these cells. The aim of this study was to investigate the effect of ۲FlgE protein present in the flagellum pod if electricity is present, and the immune system response and cells of this system, if not using the knowledge used in this study, to design a vaccine candidate and evaluate the mechanism of action. This factor is from bacteria . Culture of standard Helicobacter pylori strain and purification of bacterial genome, study of genome banks and obtaining FlgE۲ related sequences. cloning, expression and purification of recombinant FlgE۲ protein. FlgE۲ protein is purified and crystallized, the structure of which is determined by SAD method. The second third is H.pylori. FlgE۲ crystal surface projector and Cap protein FlgD regulatory protein represent complementary function between FlgE۱ & FlgE۲ proteins in bacterial flagella. The presence of two different proteins at the hook site indicates that your H.pylori structure is different. This study was performed for the first time in the world by recombinant FlgE۲ protein, the details of which are Helicobacter pylori flagellum, introduced as a candidate for vaccine and immune response against Helicobacter pylori. This study suggests that this protein may play a role in maintaining the necessary conditions under stress. But more studies are needed to confirm this hypothesis.

کلیدواژه ها:

Flagel ، FlgE۲ protein ، vaccine ، Macrophage ، cytokines