The effects of intracerebroventricular injection of Aplin-۱۳ on food intake of broiler chicks

Apelin is an endogenous peptide ligand for APJ orphan receptors. APJ is a G protein coupled receptor that is very similar to angiotensin II receptors. The apelinergic system is present in most tissues of the body, including the hypothalamus in the brain. In this study, the effect of intracerebroventricular injection of different doses of Aplin-۱۳ on food intake was investigated in Ross ۳۰۸ broilers at the age of ۵ and ۷ days. On the day of injection, ۳ hours fasting were applied to the chicks to coordinate their food intake. In this study, intraventricular injection was performed into the right lateral ventricle of chickens based on the method of Davis et al. After the injection, food was given to the chicks and food intake was measured at half, one, two, three, four, eight and twenty one hours after the injection. In order to analyze the data and draw a diagram, Prism software version was used. Data analysis was performed by independent t-test. Significance level was considered P <۰.۰۵. In ۵-day-old chicks, both doses of ۱ and ۱.۵ μg significantly reduced food intake at ۲۱ hours after injection, and also the dose of ۱.۵ μg significantly reduced food intake at ۴ hours after injection. In seven-day-old chicks, the doses of ۱ and ۴ μg of Aplin-۱۳ had no effect on food intake compared to the control group. The dose of ۲ μg also significantly reduced food intake from ۸ to ۲۱ hours after injection. The results of this study showed that Applein-۱۳ reduced food intake in ۵ and ۷ day old chicks compared to the control group, but this effect was not significant in all doses.

کلیدواژه ها:

Apelin receptor ، Hypothalamus ، Ross ۳۰۸