Effect of hydroalcoholic extract of clove (Syzygium aromaticum L.) on morphine-induced conditioned place preference in male rats

Nowadays, the use of herbs is very common and few studies have been done about their side effects. Cloves have an antinociceptive effect similar to morphine. Therefore, the aim of the present study was to investigate the effect of hydroalcoholic extract of clove on morphine-induced conditioned place preference in male rats. Twenty-four male Wistar rats weighing ۲۵۰ to ۳۰۰ g were selected and divided into ۸ groups (n = ۸) in each group (n = ۸). Healthy extract recipient at doses of ۲۵,۱۰۰mg / kg. To induce morphine dependency, mice were injected intraperitoneally for three days (morphine) ۱۰mg / kg and saline ۰.۳ cc intraperitoneally for six hours, respectively, in the light (morphine) and dark (saline) apparatus, respectively. cpp (conditioned place preference) were incubated for ۴۵ min. On the test day, the extract was tested for morphine-induced conditioned place preference half an hour before intraperitoneal injection of solvent extract or DMSO (۰.۰۳cc) and clove extract at doses (۲۵,۱۰۰mg / kg). The pro method and the cpp machine were evaluated and the time spent in the room's bright room was considered as the location preference. During all tests, the motor activity of the animals was also measured. Data were analyzed using one-way ANOVA and Tukey test (p <۰.۰۵). Results showed that ۲۵mg / kg dose of clove extract decreased (p <۰.۰۰۱), and ۱۰۰mg / kg dose of clove extract increased (p <۰.۰۰۱) the morphine-induced conditioned place preference compared to the control or receiving group. Clove extract at ۲۵mg / kg decreased motor activity (p <۰.۰۵) compared to control group. These results suggest that the hydroalcoholic extract of clove has an effect on morphine-induced psychological dependence and locomotor activity.

کلیدواژه ها:

Morphine ، Conditioned Place Preference ، Psychological Dependence ، Locomotor Activity