Identification of common factors involved in organ fibrosis using analysis of microarray dada

Fibrosis is known as a severe pathophysiological complication that can severely spoil the normal structure and function of implicated tissues. Identifying the critical genes in the occurrence of this complication in various organs can be useful in controlling and treating their fibrosis. This study aimed to identify important genes affecting fibrosis. For this purpose, three separate Microarray Datasets with access numbers GSE ۹۷۵۴۵۶ (for lung tissue), GSE۱۳۰۱۲۳ (for liver), and GSE۹۷۵۴۶ (for kidney) were downloaded from the GEO at the NCBI database, and their data was analyzed using GEO۲R. For all studies, data were analyzed utilizing the parameter P-value ≤ ۰.۰۵ and Log Fc │≥۱. The up-regulated genes of all three tissues were compared with each other, and ultimately, five common genes were identified in the fibrosis of the studied tissues (lung, liver and kidney). These five common genes were analyzed in Enrich r database .The results of the analysis of these genes showed that TAL۱, ELK۴ and NANOG were the most important upstream factors for these common genes (P-value ≤ ۰.۰۵). On the other hand, analysis of the common genes with KEGG database illustrated that they have a significant relationship with cortisol synthesis and secretion and AMPK signaling pathway (P-value ≤ ۰.۰۵). Gene ontology and biological process analysis of these genes with the Enrich r database demonstrated that these five genes are involved in different processes such as positive regulation of action potential. The findings of this study showed that common and important pathways and factors are involved in the fibrosis of studied organs. Further studies are needed to verify the results of these analyzes with further studies and samples.

کلیدواژه ها:

Bioinformatics ، Fibrosis ، Signaling pathways ، Biological processes ، Transcriptional factors