Isolation and identification of oil enhancing recovery bactery frome Oil-contaminated effluents and oil sludge in desalination tanks

Background and Aim : Oil leakage and oil crude products lead to lots of damages throughout exploitative process in the sea and the land .Therefore, world environmental protection agency and non-governmental organization supporting environment in the most of industrial countries attempted to solve this problem.Recently, it is increasingly considered to biological technology in industrial scope and these days most of industries use high potential of the organisms including petroleum although they did not used to apply biological systems This issue provides the most studies and researches in the biological industryMethods : Sampling.culture.Isolation. Gas chromatography.Surface tension measurementResults : In order to identify several samples of effluents and oil sludge from the desalination tanks of the National Company for Southern Oilfields, ۱۰ isolates were isolated using conventional selective enrichment (msm medium with crude oil). The measured surface tension and degraded hydrocarbons showed that the efficiency of theses two isolates (w۱,s۶)were better than the others. The isolates s۶ ,w۱ were observed negative by using biochemistry tests. The two isolates reproduced and sequenced ۱۶s rRNA gene to identify these isolates accurately. According to BLAST application in NCBI database and Ez taxone software ,w۱ isolate was most similar to pseudomonas alcaliphila bacteria and s۶ was most similar to pseudomonas mendocina. The supernatants of the isolate treatment in recovered culture analyzed through gas chromatography to study and to degrade aromatic and saturated compounds . Chromatic results of s۶, w۱ showed that heavy hydrocarbons degrade to light compound ,shortening chains and decreasing their peak and also degradation of n-alkane and it can call alkane degrading bacteria .Therefore, the degradation of high-weight compounds and changing them into low-weight compounds as a substrateto determine Carbone source and biosurfactant production are the most important mechanism for bacteriaConclusion : Two isolated native bacteria have high efficiencies in biosurfactant production and decomposition of petroleum hydrocarbons and growth in salt-containing medium.Therefore, it can be said that these bacteria can be used in bioremediation of oil-contaminated environments and other environmental applications and biotechnology.Also, due to the isolation of bacteria from samples containing high concentrations of salt, they are likely to show resistance to salinity stress

کلیدواژه ها:

biosurfactant ، oil hydrocarbon ، Bioremediation