Antibacterial effect of Gleditschia caspica ethanolic extraction on the Escherichia coli

Background and Aim : Escherichia coli (E. coli) strains live harmlessly in the colon and seldom cause disease in healthy individuals, a number of pathogenic strains can cause intestinal and extraintestinal diseases both in healthy and immunocompromised individuals. According to researches, Gleditschia caspica plant extracts showed activity against bacteria. In recent years, resistance to a variety of drugs has emerged in humans due to excessive use of antimicrobial drugs. The drug resistance created use of herbal medicines. The aim of this study was to determine of antibacterial effect of Gleditschia caspica alcoholic extract on the E. coli.Methods : Gleditschia Caspica plant from areas around Behshahr city (Mazandaran province, ۲۰۱۵ summer). was collected and identified in Herbalist Farabi (Tehran-Iran). Ethanolic extraction was performed by rotary evaporator. Antimicrobial effects by tubes in the concentrations of ۰.۷۸, ۱.۵۶, ۳.۱۲, ۶.۲۵, ۱۲.۵, ۲۵, ۵۰ and ۱۰۰ mg/ml of ethanolic extract were used on the Escherichia coli ATCC ۲۵۹۲۲. Then disk diffusion, MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) of extracts were determined (Positive control; Gentamicin ۳۰ μg, Negative control: dimethyl sulfoxide). For statistical analysis, SPSS ۱۶ software and two-way analysis of variance were used (p≤۰.۰۵).Results : The halo diameter was not higher than that gentamicin antibiotic (۱۵.۳ mm). Maximum diameter of the halo was ۱۱.۵ mm at the ۲۰ μl of ۱۰۰ mg/ml of concentration. MIC and MBC were in the ۲۵ mg/ml and ۱۰۰ mg/ml, respectively. According to the statistical results, with increasing the concentration of plant extract, its antibacterial effect is more (p≤۰.۰۵).Conclusion : This study is a scientific basis for using herbal extracts to treat infections that caused by strains of bacterial organisms. Due to the low price, ease of access and significant antibacterial effects of Gleditschia caspica plant extract on the E.coli, it can be considered as a plant product and natural medicine by researchers and users.

کلیدواژه ها:

Gleditschia caspica ، Antibacterial effect ، Escherichia coli ، alcoholic extract