Purification and isolation of SHV beta-lactamase enzyme from the Klebsiella pneumoniae and effect of Thyme essence on its expression by Real Time PCR and SDS PAGE methods

Background and Aim : Klebsiella pneumoniae is a gram-negative bacteria of Enterobacteriaceae family.Increasing the resistance of this bacteria to many antibiotics has caused serious problems in treatment of its infections .Today, antibiotic resistance is high in pathogenic bacteria and the number of deaths is increasing with these infections. The aim of this study is to investigate antibacterial effects and mechanism of action of Thyme essence against SHV beta-lactamase expression in Klebsiella pneumoniae strains which have a SHV gene by using Real-time PCR method.Methods : In a descriptive cross-sectional study, ۱۲ isolates of Klebsiella pneumoniae were collected. Subsequently, the presence of SHV β-lactamase gene were evaluated in all strains by using PCR method. Then, in in vitro situation, Thyme essence activity was investigated against all isolates which have SHV gene by micro broth dilution method. Then the SHV beta -lactamase expression in strains which treated with Thyme essence and non-treated strains of Klebsiella pneumoniae were evaluated by using Real Time PCR and SDS-PAGE methods.Results : The results of this study showed that ۲ isolates had SHV beta-lactamase gene. The results of the Real-Time PCR test showed that the expression of SHV gene in Klebsiella pneumoniae strains which treated with Thyme essence, was about ۴۱% lower than non-treated strains.Conclusion : Thyme essence has a strong anti-beta-lactamase activity against Klebsiella pneumonia strains which have a SHV gene. This essence can be used alone or beside the antibiotic treatments in order to have a better effect of antibiotic treatment.

کلیدواژه ها:

Thyme essence ، Klebsiella pneumoniae ، SHV gene ، Real-time-PCR