The prevalence of NetB and TpeL genes in Clostridium perfringens type A isolated from Iranian animals

Background and Aim : Clostridium perfringens is anaerobic and Gram-positive bacteria, which are found in the intestinal tract of mammals and birds. C. perfringens causes different disease in humans and animals including enterotoxaemia, lamb dysentery, avian necrotic enteritis, and neonatal haemorrhage due to their major and minor toxins. The aim of this study was to evaluate the prevalence of NetB and TpeL genes in C. perfringens type A among Iranian isolates.Methods : In total, ۶۱ archive isolates obtained from intestinal and feces samples (sheep, cattle, calf, goat and hen suspected to clostridial disease) all over the country, were subjected for detection by traditional test (microbiological and biochemical tests). Then, isolates were evaluated using polymerase chain reaction (PCR) to diagnostic molecular of CPA, NetB and TpeL genes. C. perfringens type B, C, D and C. septicum were included as the reference strains.Results : In bacterial culture ۲۷ out of ۶۱ isolates were tested positive. C. perfringens was detected in all isolates by microbiological and biochemical tests. Genotyping results showed all isolates belonged to C. perfringens type A. Eighteen and sixteen isolates (۶۶.۶۶% and ۵۹.۲%) were positive for NetB and TpeL genes respectively.Conclusion : The results indicate that the role of NetB and TpeL genes in pathogenesis and also needs to be further investigated.

کلیدواژه ها:

Clostridium perfringens ، PCR ، biochemical tests ، genotyping ، NetB ، TpeL