مقایسه روش های مختلف تشخیص بیماری لوسمی گاو

several farms were selected around Tehran in order to find epizootie of bovine Leukosis disease and a number of dairy cows were bleed randomly in these farms . . the collected. blood specimen were centrifuged and sera were separated and kept in freezer before the experiment . - The collected sera were used in immunodiffusion test using known antigen in central well . positive sera in above and below wells and test sera in the reminder . . - The sera possessing antibody were recorded and two farms with high positive cases selected for further investingations . -A total of 200 sera and milk specimen were collected from the infected farms . I I . sera tested in immunodiffusion and milk in lact Elisa tests . - The positive results were compared in the two experiments . I - 50 specimen from every province were tested in immunodiffusion test and total incidence of the disease were recorded throughout the contry .