جداسازی و تعیین هویت باکتری عضو کمپلکس مایکو باکتریوم توبرکلوزیس از گاوهای توبرکولین مثبت و جوندگان دامداریهای الوده

Bovine tuberculosis is an infectious disease of cattle caused by Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex. This chronic disease also affects a wide range of other domestic and wildlife animals and may also cause disease in humans. It is very important to control and prevent the epidemic among human and animals because of zoonotic identity of the pathogen and several cattle flocks in suburb of cities. It must be done better through more active surveillances. During this study, the causative agent of disease must be isolated from reactor cattle and also the rodents found and hunted on the reactor or suspected farms. Finally, it must be distinguished that which isolates (species) are circulated in those areas. By use of Tuberculin test samples have been selected .They consist of 866 lymph nodes of reactor cattle and 30 mice from the same farms. All samples were referred to laboratory, and then cultured in specific Lowenstein- Jensen slant media. After at least 8 weeks incubation (at 37؛c) DNA was extracted from 8 isolates of 169 lymph nodes and also 5 isolates of 30 mice to identify the isolates belonging to the Mycobacterium tuberculosis complex, by using PCR detection of insertion sequence 6110 (IS6110).According to the test all isolates (169 isolates of lymph nodes &5 isolates of mice) belong to the family of Mycobacterium tuberculosis complex. RD typing method is used to identify species. Based on obtained results, 169 isolates of reactor cattle were confirmed as Mycobacterium bovis.