Silymarin inhibits Toll-like receptor 8 gene expression and apoptosis in Ramos cancer cell line

سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 399

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شناسه ملی سند علمی:

JR_AJP-10-2_007

تاریخ نمایه سازی: 24 فروردین 1399

چکیده مقاله:

Objective: Silymarin is a herbal extract containing flavonolignans, and it has inhibitory effects against the growth of different cancer cell lines by inducing apoptosis. Toll-like receptors are suggested as a novel and attractive target to treat cancer. The current study aimed at examining the mechanism of silymarin-induced apoptosis in Ramos cells and investigating its effects on TLR8 expression. Materials and Methods: The half maximal inhibitory concentration (IC50) of silymarin in Ramos cells was determined via MTT viability test while the type of cell death was tested by annexin V/propidium iodide (PI) double staining method. The activity of caspase-3 and expression of TLR8 were measured in a time-dependent manner (in IC50) by colorimetric assay and real-time polymerase chain reaction (RT-PCR), respectively. Results: The results of MTT showed that IC50 of silymarin in Ramos cells was 100 μg/ml after 48 hr treatment (p<0.01). Flow cytometry by annexin V/PI, showed that silymarin induced early/late apoptosis in this cell line (p Conclusion: The results indicated a new mechanism in the anticancer activity ofToll-like receptor (TLR) signaling after silymarin treatment in Ramos cancer cell line. This plant could be used to develop anticancer agents inhibiting TLRs.

نویسندگان

Nasrin Ranjbar

Student Scientific Research Center, Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.|Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences,

Ramin Saravani

Cellular and Molecular Research Center, Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.|Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences,

Zohreh Faezizadeh

Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran

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