HN-00200070_Association of Proinflammatory Genes Expression with Serum Interleukin 1β and Free Fatty Acids in Metabolically Healthy and Unhealthy Abdominally Obese Individuals: A Case-Control Study

Introduction: Proinflammatory genes are highly expressed in several metabolic disorders associated with obesity. The relationship between gene expression and downstream inflammatory markers and metabolic state is unclear. The aim of this study was to compare Toll-Like Receptor 2 (TLR2), Myeloid Differentiation Factor 88 (MyD88), and NFĸB mRNA expression levels between metabolically healthy abdominally obese (MHAO) and metabolically unhealthy abdominally obese (MUAO) individuals.Methods: In this case-control study, MUAO (n=36) and MHAO (n=34) groups were recruited. mRNA expression of the genes was assessed in freshly isolated peripheral blood mononuclear cells (PBMCs), using quantitative real-time PCR (qPCR). FFAs and IL-1β were measured by ELISA.Results: Serum FBS, TG, HDL-C, systolicand diastolic blood pressure were significantly higher in MUAO compared to MHAO groups (p < 0.05). The odds of MUAO was significantly decreased with high HDL-C (OR = 0.22, 95%CI: 0.08–0.63) andincreased with high FBS (OR = 7.04, 95%CI: 1.42–34.69) and TG (OR = 30.55, 95%CI: 7.48–60.67). There were no significant differences in proinflammatory genes, serum FFAs and IL-1β between groups. No associations were found between the genes expression and serum markers. NFĸB expression was significantly correlated with TLR2 and MyD88 (r = 0.747; p < 0.001). There was a significant correlation between TLR2 and MyD88 expression and between serum FFAs and IL-1β in each group (p < 0.001).Conclusion: Serum IL-1β, FFAs, and mRNA expression of TLR2, MyD88, and NFĸB may be due to abdominal obesity and not the presence or absence of metabolic health.

کلیدواژه ها:

Toll like receptor2 ، Myeloid Differentiation Factor88 ، Nuclear factor kappa B Interleukin-1beta ، abdominal obesity