Evolution and their expression of rs738409 in patients with nonalcoholic fatty liver disease (NAFLD)

Introduction : Fatty liver (hepatosteatosis) is the earliest abnormality in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) and alcoholic fatty liver disease (AFLD) In fact, the liver has a little fat always, but if it becomes over 10 percent of liver volume AFLD and NAFLD could be caused. nonalcoholic fatty liver disease (NAFLD) is a lot more common than you think. There is no single reason or cause as to why so many suffer from this disease. Most often is a mix of different causes. is exposed in the presence of other risk factors, such as severe obesity, visceral adiposity, increased intake of sugars or omega-6 poly-unsaturated fatty acids, and other genetic factors. This study aimed to investigate the relationship single polymorphism PNPLA3I148M in patients with NAFLD and compared the results with .those reported in other parts of the world setsMethod: DNA was extracted from the peripheral blood of 85 patients with nonalcoholic fatty liver disease and 120 normal persons. 356 bp gene fragment was amplified by the polymerase chain reaction (PCR). subsequently, the mutation was .screened for random fragment length polymorphism analysis (RFLP) Results: Statistical analysis showed that the risk allele (G-allele) frequency of rs738409 was 40.07 % in the control subjects and 80.12 % in patients with NAFLD; this shows a strong association PNPLA3I148M polymorphism with the non-alcoholic .fatty liver disease in our population (P-value <0.001 OR = 3.651, CI: 2.123 - 4.471) .

کلیدواژه ها:

Nonalcoholic fatty liver ، NAFLD ، RFLP PCR ، polymorphism