Evaluation of Antimicrobial Resistance and Immune Evasion Cluster Genes in Clinical Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Khuzestan Province, Iran
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 315
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شناسه ملی سند علمی:
JR_IEM-5-1_002
تاریخ نمایه سازی: 3 اسفند 1398
چکیده مقاله:
Aims: Methicillin-resistant Staphylococcus aureus (MRSA) is recognized as an important health problem worldwide. To counteract the human innate immunity, S. aureus produces a number of immune evasion clusters (IEC) including staphylokinase (SAK), staphylococcal enterotoxin P (SEP), staphylococcal enterotoxin A (SEA), staphylococcal complement inhibitor (SCIN), and chemotaxis inhibitory protein (CHIP), encoded by sak, sep, sea, scn, and chp genes, respectively. These genes are carried by β-haemolysin-converting bacteriophages. The present study was conducted to determine the IEC phage types and antibiotic resistance patterns in 145 clinical MRSA isolates from Khuzestan Province, Iran. Materials & Methods: All the isolates were investigated by disc diffusion method and PCR assay for sak, sep, sea, scn, and chp genes.Findings: The assessment of antibiotic resistance showed the highest resistance rate towards penicillin (97.25%), followed by methicillin (95.8%), ceftazidime (81.4%), erythromycin (71.8%), clindamycin (61.4%), ciprofloxacin (60.7%), gentamycin (56%), imipenem (56.55%), and vancomycin (0%), respectively. Also, the frequency of IEC types was as follows: Type A, 4.8%; Type B, 9%; Type C, 13.1%; Type D, 12.4%; Type E, 27.6%; Type F, 1.4%; Type G, 0.7%; and Type H, 6.9%. On the other hand, 24.1% of the isolates showed no IEC type. Conclusion: The findings showed that IEC-carrying bacteriophages were highly prevalent among the MRSA strains, resulting in the adaptation and counteraction of bacteria with the human immune system. Therefore, understanding the role of IEC in bacteria virulence can improve our knowledge about the evolution, vaccination, and treatment of S. aureus infection.
کلیدواژه ها:
نویسندگان
s Ariyarad
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
s.e Rezatofighi
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Biotechnology and Biological Science Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran
h motamedi
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Biotechnology and Biological Science Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran