Anti - whole Influenza A/H1N1 Virus Polyclonal Antibody for passive immunization against influenza

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 568

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شناسه ملی سند علمی:

MEDISM20_351

تاریخ نمایه سازی: 26 بهمن 1398

چکیده مقاله:

Introduction and Objectives: Acute respiratory viral infections are the main cause of outbreaks all over the world. The efficacy of medical interventions such as vaccination is generally failed due to antigenic shifts and drifts among virus strains and the lack of long-lasting effective immune responses. Following the recent influenza virus pandemic, and the absence of enough efficacious vaccines for prevention, it is an outmost need to have access to strategies which provide enough periods to prepare a new vaccine. One of the practical ways to prevent the spread and prevalence of the viral diseases is intranasal passive immunization using antiviral antibodies. Materials and Methods: Influenza virusA/H1N1/PR8 was inactivated using UV radiation and then inactivated whole virus with Freund’s adjuvants (Complete and Incomplete) was injected into New-Zealand white male rabbits. The polyclonal antisera of rabbits were evaluated by HI. Then IgG was purified using DAEA-cellulose column chromatography. The quality and properties of purified IgG were evaluated using SDS-PAGE and ELISA. Finally, using Virus Neutralization Test (VNT), virus capturing using antibody was evaluated. Results: The HI result showed that the produced anti-whole virus antibody was able to recognize whole virus epitopes. The HI results confirmed anti-virus pAb reached reasonable titers after three injections. The Purified polyclonal IgG was evaluated using ELISA and VNT. The results showed IgG reacted with the virus antigen up to 1:32000 dilution in ELISA and 2048 titer in VNT. Conclusions: The data showed that inactivated whole virus was able to stimulate immune response to produce antibody at satisfactory level.

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نویسندگان

Behrokh Farahmand

Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran

Fatemeh Fotouhi Chahouki

Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran

Parvaneh Mehrbod

Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran

Fatemeh Eskandari

Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran

Mehdi Alikhani

Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran

Ali Torabi

Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran