T cell-mediated immune responses of chicken inoculated with fowl pox vaccine

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 349

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شناسه ملی سند علمی:

MEDISM20_137

تاریخ نمایه سازی: 26 بهمن 1398

چکیده مقاله:

Introduction and Objectives: Fowl pox is a viral disease that is widely distributed throughout the world. The disease has an economic impact on the poultry industry, and even the outbreak has been reported in vaccinated flocks. This study was designed to evaluate the T cell-mediated immune responses of chickens induced by fowl pox (FP) vaccine. Materials and Methods: Three groups of 40 non-pathogenic 21-day-old chicks were used. They were vaccinated with either the local or commercial FP vaccines. Peripheral blood mononuclear cells were isolated and the percentage of CD3+, CD3+ CD4+ and CD3+ CD8+ T lymphocytes were analyzed by flow cytometric method. The concentration of inflammatory cytokines (IFN-γ) and anti-inflammatory (IL-4) in peripheral blood mononuclear cells were also measured using the ELISA method. Results: The results showed that, after 7 days post immunization, a maximum (90-100%) swelling formation ( take ) on the site of vaccinated chickens were observed. The ratios of CD4+ to CD8+ T-lymphocytes in both groups of commercial and local FP vaccinated groups were significantly higher (P < 0.05) than the control group. The percentages of CD3+, CD3+CD4+, and CD3+CD8+ T-lymphocytes were increased (P < 0.05) in vaccinated chickens with commercial and local FP vaccines. The results of cytokine analysis showed that the ratio concentration of IFN-γ to IL-4 in supernatant cell culture of vaccinated chickens in both groups were higher than the control group. The results show that the flow cytometric method and the cytokine assay are appropriate for measuring IFN-γ and IL-4 by T-cells (CD4+ and CD8+). Conclusion: This study revealed that the protective immunity may be associated with increased cellular immunity (predominantly Th1 type-reaction), enhancing the proliferation of T cells and increasing CD4+ to CD8+ ratios due to vaccination of the FP vaccine.

کلیدواژه ها:

FP vaccine ، IFN-γ ، flow cytometry ، CD4 and Cd8 T-cells

نویسندگان

Mohammad Reza Azizi

Department of Microbiology, Faculty of Sciences, Islamic Azad University, Karaj Branch

Esmaeil Asli

Razi Vaccine and Serum Research Institute. Agricultural, Research Education and Extension Organization (AREEO), Karaj, Iran

Ali Mohammad Behroozikhah

Razi Vaccine and Serum Research Institute. Agricultural, Research Education and Extension Organization (AREEO), Karaj, Iran

B. Khalesi

Razi Vaccine and Serum Research Institute. Agricultural, Research Education and Extension Organization (AREEO), Karaj, Iran