Prevalence assay of quinolone resistance among Enterobacteriaceae isolated from urban and hospital wastewater in Karaj

Introduction and Objectives: Hospitals are hotspots for antimicrobial-resistant bacteria (ARB) and will be ejected from hospitals via wastewater systems. In this study, we investigated the prevalence of quinolone resistance in Entrobacteriaceae strains isolated from hospitals and urban wastewater in Karaj. Materials and Methods: A total of 60 Entrobacteriaceae strains isolated from hospitals and urban wastewater in Alborz province during the spring of 2018. Bacterial strains were identified by standard microbiological and biochemical tests. The antimicrobial susceptibility test to ciprofloxain, levofloxacin, ofloxacin, norfloxacine and nalidxic acid was determined according to Kirby Baur assay. Results: Among the organisms cultured, Escherichia coli (70%) was the most common organism followed by Citrobacter freundii (11.66%) and Citrobacter diversus (5%). Antibiotic resistance pattern were observed as follows: nalidxic acid 71.66%, norfloxacine 28.33%, ciprofloxain 26.66%, ofloxacin 23.33% and levofloxacin 20%. Conclusion: The wide geographic spread of antibiotic-resistant strains may be connected with their transmission between hospitals, urban wastewater and environment. Antimicrobial resistance should now be seen as an environmental pollutant and new wastewater treatment processes must be assessed for their capability in eliminating antimicrobial-resistant bacteria, especially from hospital effluents.

کلیدواژه ها:

Entrobacteriaceae ، quinolone resistance ، wastewater