Diosmin alleviates bleomycin-induced lung injury in mice

Background: Pulmonary fibrosis (PF) is a fatal and incurable lung disease. Bleomycin (BLM) as an antitumor antibiotic that causes lung fibrosis in human patients has been used widely to develop animal models of PF. Diosmin (Dio) is a safe flavonoid with good tolerability and low toxicity.Objective: The purpose of the current study was to evaluate the effects of Dio on BLM-induced PF.Methods: PF model was induced by BLM (3.5 mg/kg, intratarchealy (i.t.) in NMRI albino mice. The animal divided into 2 sections, each consists of 4 groups. Section 1 treated for 3 days and section 2 treated for 21 days. The control group was administered 2ml/kg normal saline,i.t., the BLM group received single dose of BLM, i.t., groups 3 and 4 received Dio 50 mg/kg and Dio 100 mg/kg, respectively three days before BLM and continuing it for 3 or 21 days by oral gavage. The animals were sacrificed 24 h after the last administration, then, lung index and oxidative stress markers were measured in lung tissue. Histopathological changes were evaluated by microscopic examination of stained sections with hematoxylin-eosin and Masson s trichrome.Results: BLM significantly induced oxidative stress and inflammation by increase the level of malonedaldehyde (MDA) (p<0.001) and decrease the level of glutathione (GSH) and catalase activity in the lung (p<0.01 and p<0.05, respectively). Dio 50 mg/kg significantly increased GSH level, CAT activity and reduced MDA level (p<0.05). In addition, Dio improved histopathological injuries in hematoxylin and eosin and Masson s trichrome stained sections.Conclusions: These findings suggest that Dio 50 mg/kg has protective effects against BLM -induced lung injury, which may be due to its antioxidant, anti-inflammatory and antifibrotic effects.

کلیدواژه ها:

Bleomycin ، Pulmonary fibrosis ، Oxidative stress ، Diosmin ، Mice