Expression of a halostable phytase from Nesterenkonia sp.strain F in Escherichia coli

Phytases hydrolyse phytate (myo-inositol hexakisphosphate), the principal of phosphate stored in plant seeds to produce phosphate and lower phosphorylated myo-inositols. The gene encoding phytase from Nesterenkonia sp. strain F was cloned, sequenced and expression in Escherichia coli. Expression was induced by IPTG at a concentration of 0.5 mM. Enzyme activity was performed in the presence of phytic acid. the optimal PH and temperature of the enzyme were PH 6.5-7.5 and 20˚C , respectively. Genetic cloning reveals that the protein of this enzyme consist of 441 amino acid with a calculated molecular weight of 48 KDa . A higher enzyme activity was obtained when the gene expression was done in the presence of NaCl.. We have successfully expressed the cloned gene in Escherichia coli from its putative initiation codon, showing that the gene actually encodes the phytase. Due to its pH profile and optimum, it could be an interesting candidate for Various application.