Detection and Functional Analysis of Calumenin-Related MicroRNAs in a Colorectal Cancer Cell Line, SW480

CREC is a family of calcium-binding proteins, with multiple EF-hand motifs, that are widely distributed in different cellular compartments including; nucleus, cytosol, and endoplasmic network, etc. One of the member of this protein family is Calumenin (CALU), for which alteration in expression pattern is reported in cancer and a number of other diseases. However, the underlying signaling pathways for its regulation has not been elucidated yet. Considering the key role of miRNAs in cancer progression, we aimed to detect the miRNAs that are associated with the CALU regulation. With this aim, the miRbase and miRmap algorithms were used to identify the CALU related miRNAs, which led to detection of Mir30 family as the most relevant microRNAs which are related to calumenin with high scores.Taking a quantitative RT-PCR approach, as an in vitro invasive cancer model, the levels ofidentified miRNAs were evaluated in a colorectal cancer (CRC) SW480 cell line. Taking advantage of the miRanda database in the subsequent step, the most upregulated and downregulated signaling pathways, correlated with CALU, were designed.The obtained data will be applicable in identification of the signaling intermediates that have crucial roles in cancer development, towards adopting suitable therapeutic strategies.

کلیدواژه ها:

Calumenin ، Colorectal cancer ، microRNA