Relationship between the expression level of miR-135a and the HOXA10 gene in eutopic and ectopic endometrium

Background: The study of microRNA expression changes, can effective in diagnosing and treating the disease. miR-135a is one of the most important micro-ribonucleic acids involved in endometriosis, which is associated with endometriosis. Among the genes that become the target of the miR-135a and are subjected to changes in the endometrium of patients with endometriosis is the HOXA10 gene. The HOXA10 gene is expressed in the endometrium in respond to steroid hormones. Objective: The aim of this study was to evaluating the expression of miR-135a and its relationship with the level of HOXA10 gene expression in both endometrial ectopic and eutopic tissues in patients with endometriosis. Materials and Methods: In this study, both case-eutopic and case-ectopic tissue samples were obtained from 17 women with endometriosis and the eutopic tissue was also sampled from 17 non-infected women as the control group. The genes expression of miR-135a and HOXA10 were investigated using q-RT PCR. Results: The significant decrease in the expression of HOXA10 gene detected in case-eutopic during the luteal phase compared to the control samples, while in the case-ectopic, the expression of this gene increased. Also, the expression miR-135a in the luteal phase of patient samples compared to controls showed a remarkable increase in the case-eutopic as well as significant decrease in the case-ectopic. Conclusion: Considering the inverse relations between the over-expression of miR-135a and the reduction of HOXA10, it concluded that miR-135a can be applied as an endometrial diagnostic and therapeutic biomarker.

کلیدواژه ها:

Endometriosis ، Gene expression ، Micro-ribonucleic acid ، HOXA10 ، miR-135a