The study two SNP of GRIN1 gene associated with addiction to heroin and methamphetamine

Introduction: Glutamate increases the probability of drug relapse. N-methyl-D-aspartate receptor (GRIN1) is a glutamate ionotropic receptor and is found in mammals brain. This study sought to investigate the association between rs11146020, rs1126442 and addiction to heroin and methamphetamine in Iranian male. Materials and methods: 90 males addicted to heroin and methamphetamine and 100 healthy male were the participants of the study. Genomic DNA extracted from the blood, and then PCR-RFLP and T-ARMS PCR were respectively used to determine the rs1126442 and rs11146020 polymorphism genotype.Results: Two SNP studied in the development of heroin and methamphetamine addiction which, in the study is not a contributing factor, because was no association between rs1126442 and rs11146020 polymorphism. The genotype frequencies of CC, GC and GG at the rs 11246020 polymorphism were 57%, 30%, and 3% in the patient, respectively. The genotype frequencies of AA, GA and GG at the rs1126442 were respectively 52%, 28%, and 10% in the patient. The results obtained from the analysis of rs1126442 and rs 11146020 polymorphism showed a significant association between the two polymorphisms with marital status and educational level and showed no association with job status. Conclusion: The results of the study indicated that neither of the two polymorphism in GRIN1 gene had not showed significant association in the control samples and addicted person.

کلیدواژه ها:

rs1126442 ، rs11146020 ، GRIN1 ، heroin ، methamphetamine