Study of miR-202 and the expression of AKAP4 gene in Patients with ductal carcinoma breast cancer as possible candidate cancer biomarker

Introduction: Breast cancer is the most common diagnostic cancer among women around the world. More reports on breast cancer suggest that early diagnosis is important for improving clinical outcomes in patients with breast cancer. In this context we investigated the expression of a cancer testis antigen (AKAP4) and miR-202 that is a miRNA with features characteristic of a CT antigen.Material and Methods: To evaluate the expression of AKAP4 and miR-202 genes، Real Time RT-PCR by SYBR green dye was used at the presence of beta-actin housekeeping gene as internal control. The expression was assessed in the blood and tissue of breast cancer patients in comparison with normal controls in 110 samples.Results: In this study we aimed to investigate the presence of AKAP4 and upregulation of miR-202 expression in breast cancer tumor tissues and its subsequent detection in peripheral blood could be considered as a possible biomarker for breast cancer diagnosis even at the early stages.Conclusion: AKAP4 gene and miR-202 expression analysis in tissue could be considered as possible early diagnostic biomarkers in breast cancer that is an urgent need for better management of cancer.

کلیدواژه ها:

AKAP4 ، miR-202 ، expression ، diagnostic biomarkers