Analysis of HLA haplotypes association in Multiple Sclerosis patients from Khuzeatan province.

Multiple sclerosis is neurodegenerative disease results from the interaction between both genetic and environmental factors. HLA genes have been identified to be involved in MS susceptibility. The HLA gene variants are associated with some autoimmune disease. Our aim was to study the association of this haplotypes in MS patients from Khuzeatan province.Methods: In this case-control study, the association of HLA alleles was investigated in 200 multiple sclerosis patients from Khuzestan province (168 female and 32 male), with female-male ratio about 4:1. HLA-typing was performed by polymerase chain reaction with sequence-specific primers (PCR-SSP).Results: we find that some haplotype of HLA have significant association in MS patients such as HLA-A*03(46.5 % patients vs. 29.5%controls, p-value:0.000 ), &HLA-DRB1*1501(41.5% vs. 22.8%, p-value:0.001); while two haplotypes have no significant association: HLA-DQB1*0602 (61.5% vs. 64%, p-value:0.605) and HLA_DRB5*01(27.72% vs. 21.39% , p-value:0.148), and two haplotypes have negative association in MS patients: HLA-A*02 (29.5% vs.54%, p-value:0.000) & HLA-DQA1*0102(60% vs. 73%, p-value:0.006).furthermore, we analyzed the haplotype map of this patients and find that 20 % of patients have two significant haplotypes (HLA-A*03, and HLA-DRB1*1501)together.Conclusion: confirmed other studies, HLA-A*03, and HLA-DRB1*1501 alleles can increase the risk of MS in khuzestan population, and HLA-A*02 and HLA-DQA1*0102 alleles probably have protection function and can reduce the risk of MS. We think that HLA haplotypes can use as a primary marker for distinguish of MS susceptibility.

کلیدواژه ها:

Multiple sclerosis ، genes ، HLA-typing.