Molecular epidemiology and genetic proximity of Human Papillomavirus by the PCR-sequencing method in the women of Qom city

The genetic polymorphisms and genetic evolution of human papillomavirus (HPV) as predictive markers of cancer as well as epidemiological studies of genital HPV infection in the general population, will play a significant role in order to plan, determine the strategy of health and promote the level and development of healthcare and treatment. This study was conducted for the first time in Qom Province, considering the risk factors associated with HPV, which it was able to analyze genetic evolution in genotypes.486 Pap smear samples were tested for HPV DNA, positive samples were sequencing and submission in the GenBank (MG825048-61). Then, after alignment phylogenetic and polymorphism analysis was performed on sequenced samples with a number of GenBank sequences.Out of 486 married women who studied, 57 (11.7%) have been infected with the HPV virus The results of sequencing showed that the genotypes 6, 11 and 61, as well as 16, 51 and 58, were the high-risk and low-risk genotypes, respectively.Investigating the genetic polymorphism of the selected region in different genotypes of the HPV showed 72.5% genetic variation, most of the nucleotide changes were singleton, non-informative, and only 36.2% of the changes led to altered amino acid but ultimately, the virus has kept its essence. In the case of phylogeny analysis, the trees plotted in both methods of Neighbor-Joining and Maximum Likelihood showed the same topology and were able to firmly separate the different genotypes of HPV.This is the first molecular epidemiology study that describes genetic polymorphism and phylogenetic analysis of HPV in Qom province.

کلیدواژه ها:

Human papillomavirus ، polymorphism ، genetic evolution ، molecular epidemiology ، Qom