The use of phosphinothricin resistance as selectable marker for Nicotiana tabacum engineering

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 382

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شناسه ملی سند علمی:

CIGS15_347

تاریخ نمایه سازی: 13 بهمن 1398

چکیده مقاله:

Phosphinothricin (PPT) is the active component of a family of environmentally safe, nonselective herbicides. The bar gene conferring tolerance to the herbicide phosphinothricin (PPT) encodes phosphinothricin acetyltransferase (PAT). Phosphinothricin Acetyl Transferase that detoxifies Phosphinothricin (PPT), the active ingredient of herbicides such as BASTA. We report here a transformation procedure with the bar gene as a selectable marker established via Agrobacterium- mediated transformation. It was introduced into the tobacco genome at a targeted site by homologous recombination. Transgenic plants of Nicotiana tabacum were regenerated by leaf disc transformation using a bar gene as a selectable marker. Transgenic plantlets were selected in medium supplemented with PPT (up to 0.1 mM). The transgenic N. tabacum plants resistant to PPT were positive upon PCR by bar gene specific primers. The transformed plants transferred to a greenhouse proved to be resistant to 1% PPT. The technology demonstrated here could perhaps be usefully transferred to other crop species.

نویسندگان

Maryam Jamshidnia

Sari Agricultural Sciences and Natural Resources University, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Sari, Iran.Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental H

Sayed Kamal Kazemitabar

Sari Agricultural Sciences and Natural Resources University, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Sari, Iran

Christian Lindermayr

Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany

Hamid Najafi Zarini

Sari Agricultural Sciences and Natural Resources University, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Sari, Iran