Using Systems Biology Approaches to Discover DEGs and Regulatory Factors Responsible for Glioblastoma

In-silico studies with reduced time and expenses has facilitated disease treatment more than before. In the current study, we have made comparisons between expression profile of monocytes obtained from Glioblastoma patients and those of healthy ones provided by GSE77043 dataset in GEO. After normalizing the data by RMA algorithms, differentially expressed genes (DEGs) were identified by adjusted p-Value and logFC parameters. JASPAR PWMs database was then used for finding transcription factors (TFs). Following performing Enrichment analysis, Gene Ontology and the affected pathways revealed by GO and Reactome databases, miRNAs regulating our DEGs were selected by miRTarBase and mapping DEGs on brain performed by Allen Brain Atlas. Our study suggests E2F1, PCBP1, TCFAP2A, WT1 and SP1 as TFs are involved in Glioblastoma condition. The ontology revealed theses DEGs are mostly involved in negative regulation of transcription from RNA polymerase II promoter and I-kappaB phosphorylation. The top3 pathways affected by them are Hemostasis, Cellular responses to stress and Signaling by Rho GTPases. Our results introduce miR-17-5p, miR-93-5p and miR-20a-5p as important miRNAs regulating gained DEGs. Mapping on the brain showed DEGs’ existence mostly in Anteromedial visual area, layer 6a and layer 3 of PCx. In conclusion, TFs identified by systems biology approaches are reasonable targets and even shortcuts in research fields. Although miRNAs can be key and new regulators for controlling Glioblastoma condition.

کلیدواژه ها:

Glioblastoma ، systems biology ، TF ، miRNA ، microarray