Evaluation the effectiveness of EST-based markers in separating of Aegilops codata species wild accessions

Knowledge of genetic diversity level for plants germplasm and the determination of genetic relationships of breeding plant materials is the basis of many plant breeding programs. In this research, three EST based markers were used to study the genetic variation among eight wild-relatives of wheat belonging to Aegilops codata species. The allelic pattern was scored on the basis of presence (1) and absence (0) bands, and the total number of 78 bands was identified. Cluster analysis and drawn dendrogram based on UPGMA method using dice similarity coefficient were divide the accessions into four groups with the high co-phenetic coefficient (0.968). In the first and third groups, three accessions in each group and in the second and fourth group only one accession were placed in each. The highest genetic similarity was observed between the IUGB-00304 and IUGB-00815 accessions. The calculated genetic indices including Na (1.5), Ne (1.39), I (0.33), He (0.22), UHe (0.23) showed a high level of diversity among the accessions. The average of polymorphism for the studied primers was 60.26%, and the highest polymorphic information was obtained for M1+Mir398 combination (PIC=0.934) and the lowest for the M2+Mir398 combination (PIC=0.861). The Principal Coordinate Analysis (PCoA) had also clearly confirmed the separation of the accessions, indicating that the EST based markers could well differentiate the Aegilops codata accessions.

کلیدواژه ها:

EST markers ، Genetic variation ، Wild-relatives of wheat