The effects of policosanol on memory and learning impairment and total thiol group (TTG) in male rats with Alzheimer’s disease induced by β-amyloid (1-40)

Background and Aim : Alzheimer s disease (AD) is a neurodegenerative disorder that recognized with progressive cognitive function failure which determined by accumulation of beta amyloid (Aβ) in extracellular space. Aβ stimulate kinds of active oxygene and causes oxidative stresses and apoptosis. Policosanole (PCO) is a reducing lipids complement has antioxidant and anti inflammatory activity effects. In this study the PCO effects on memory impairment were investigated in AD rats were induced with in AD rats.Methods : In this study 60 male Wistar rats were used. The animals were divided in 6 groups (n=10): Control, Sham (Aβ Solvent, intraventricular microinjection), AD (Aβ intraventicular microinjection), Acacia Gum (50mg/kg, 8 weeks gavaged), PCO (50mg/kg, 8 weeks gavaged) and AD with PCO ( 50mg/kg, 8 weeks gavaged). To determine cognitive dysfunction, we evaluated memory and learning process with Barnes maze test and the novel object recognition in the experimental groups. After behavioral evaluation, serum TTG was evaluation using specified kit. data were analyzed by SPSS. Statistical significance was set at p≤0.05.Results : The results of the Barnes test on training days showed that the elapsed time has increased to find the target hole in the AD group compared to the control group. The results of this study showed that intraventricular injection of A caused reduces diagnostic memory. PCO causes recovery spatial memory and diagnostic memory. In addition, serum total thiol group (TTG level) in PCO group was significantly greater versus AD group.Conclusion : Our results demonstrate that PCO has neuroprotective effects and can protect of memory with hypo lipidemic and antioxidant activity.

کلیدواژه ها:

Alzheimer s disease ، Policosanole ، memory and learning ، TTG ، Rat