Effect of intravenous administration of Apelin-13 on neural death and brain infarct volume extention after brain ischemia in male wistar rats

Background and Aim : Stroke is still one of the most important causes of death in the world that until now, no effective treatment has been found for it. Neuronal death due to stroke is one of the major causes of enhancement of ischemic region and its complications. So far, several studies have investigated the effect of intraventricular injection of Apelin-13 on cerebral ischemia but this method does not have a clinical application. In this study we aimed to evaluated the effect of intravenous injection of Apelin-13 on post-stroke brain injuryMethods : forty eight adult male Wistar rats randomly divided into 6 groups: sham, ischemia, vehicle (ischemia+ PBS) and tree treatment groups (received Apelin-13 in doses of 10, 20 & 40μgr/kg intravenously 5 minute before reperfusion ) . Brain ischemia was induced by middle cerebral artery occlusion (MCAO) for 60 minutes. stroke volume and neural death were evaluated in term by image J software and tissl staining twenty four after brain stroke induction.Results : The results of this study showed that neural death in the ischemic groups were significantly upper than the sham group (p<0.000). Treatment by apelin-13 significantly reduce neural death in all treatment groups in comparison with ischemic group (p<0.001). Also apelin-13 in doses of 20 and 40 gr/kg could significantly decrease infarct volume in comparison with ischemic group (p<0.01).Conclusion : Intravenous administration of apelin-13 could decrease neural death and infarct volume that resulted from brain ischemia in male wistar rats. However, further studies are needed to understand the mechanisms underlying Apelin-13 administration intravenously affects on brain ischemia and also to identify its possible complications.

کلیدواژه ها:

Intravenous ، Apelin-13 ، Brain infarct volume ، Neural death