Investigation of the bfpGene Frequency in Acinetobacters Resistantto Aminoglycosides and Carbapenems with the Use of PCR Among Patients Admitted to Intensive Care Units and Burn Unitsin Sari ‚ Iran

Introduction and objectives: Biofilms are surface-bound colonies of bacteria bound to the abiotic surfaces in an extracellular extensive exo polymeric matrix which are associated with many chronic infections among hospitalized patients .Multidrug resistant strains of Acinetobacter baumannii (MDR-AB) have emerged as an important cause of hospital-acquired infections among patients admitted to Intensive Care Units and burn units. The aim of thisresearch was to study the frequency of the bfp gene in Acinetobacterbaumannii strains resistant to Aminoglycosides and Carbapenems in Sari‚ Iran. Materials and Methods: In this research, 100 A. baumannii isolates from patients admitted to Intensive Care Unit and burn units in Sari‚ Iran during 2017, were tested for their resistance to Aminoglycosides and Carbapenems by Disk Diffusion, according to the CLSI protocol. In the next step, the bfpgenes frequency in antibiotic-resistant strains were detected by the PCR method. Results and Conclusion: This study showed that of the 100 Acinetobacter baumanniiisolates, 63% (63) were Resistant to Aminoglycosides and Carbapenems andamong themthe bfpgene (biofilm producer) was detected in 32(52%) isolates. This is the first study which shows the bfp gene frequencyin Acinetobacter baumannii strains resistant to Aminoglycosides and Carbapenems in Iran.