The first Investigation of the phylogenetic Groups of Pathogenic E. coli Strains Isolated from Urinary Tract Infections in Patients Referred to Hospitals in Hamedan

Background and Objectives: Urinary tract infection is one of the most common infections which is the second common after respiratory tract infections. Escherichia coli is the most prevalent cause of UTI in 90-80% of patients in all age groups. The most important known phylogenetic groups of these bacteria are the groups A, B1, B2, D. Phylogenetic variation is due to differences in geographical conditions, lifestyle, antibiotic use pattern, antibiotic resistance, growth rate and pathogenicity of bacteria. The aim of the present study was to investigate the phylogenetic grouping of E.coli strains isolated from urinary tract infections for further controlling program of urinary tract infections.Materials and Methods: In this cross-sectional descriptive study, 70 Escherichia coli isolates were collected from patients with urinary tract infection admitted to Hamadan hospitals. After confirmation of the isolates by biochemical and molecular methods, multiplex PCR and specific primers of chu, yjaA and TSPE4.C2 fragments were used for typing and phylogenetic grouping of Escherichia coli strains. Results: Of the 70 Escherichia coli identified, 56 (80%) samples were for women and 14 (20%) for men. Phylogenetic groups evaluated revealed 34 (48.58%) strains in group B2, 24 (34.28%) in group D, 9 (12.86%) in group A and 3 (4.28%) belonged to group B1. Conclusion: Escherichia coli isolated from urinary tract infections in this area belonged mostly to the B2 phylogenetic group, which is consistent with most of the international research.