Curcumin-Loaded Mesoporous Silica Nanoparticles/Polycaprolactone-Gelatin Nanofiber Composites Enhanced hTERT Gene Expression in Human Adipose Derived-Stem Cells

Background and Aim: This study aimed to develop a drug carrier based on amine-functionalized mesoporous silica nanoparticles (MSNs) loaded into polymeric nanofibers for efficient and sustained delivery of a poorly water-soluble drug, curcumin, and to study its effects on human telomerase reverse transcriptase (hTERT) expression levels as a hallmark of aging in human adipose tissue-derived stem cells (hADSC). Methods: For this purpose, Curcumin-loaded MSNs /Polycaprolactone-Gelatin nanofiber composites were successfully fabricated by optimizing the electrospinning parameters and characterized using FE-SEM, TEM, FTIR and UTM. hADSCs were isolated from adipose tissue and their viabilities on Curcumin-MSNs loaded into Polycaprolactone-Gelatin nanofiber were assessed by MTT. hTERT expression and percentage of senescent cells were evaluated using real-time PCR and senescence-associated β-galactosidase (SA-ß-gal) activity, respectively. Results: The results demonstrated significant proliferation of hADSCs on Curcumin-MSNs loaded Polycaprolactone-Gelatin nanofiber after 14 and 28 days compared to Curcumin loaded Polycaprolactone-Gelatin and neat Polycaprolactone-Gelatin nanofibers. In addition to, SA-ß-gal staining showed that Curcumin-MSNs loaded Polycaprolactone-Gelatin nanofiber meaningfully reduced the number of senescent cells after 14 and 28 days. Moreover, hTERT expression levels significantly enhanced in the cells seeded on Curcumin-MSNs loaded Polycaprolactone-Gelatin nanofiber than Curcumin loaded Polycaprolactone-Gelatin and neat Polycaprolactone-Gelatin nanofibers group (P<0.001). Conclusion: As a conclusion, Curcumin loaded amine-functionalized mesoporous silica nanoparticles embed in polymeric nanofibers may have an advantageous potential to enhance the lifespan of hADSCs through promoting hTERT gene expression.

کلیدواژه ها:

Curcumin ، Mesoporous Silica Nanoparticles ، hTERT ، hADSCs