Design and preparation of solid phase microextraction based on molecular organic framework for use in bioanalysis

Due to the nontoxic and highly porosity, MOFs based on iron(III) was applied as sorbent. The Fe3O[C6H4(CO2)2]3.nH2O is constructed from oxygen-centered iron(III) carboxylate trimer molecular building blocks, which are linked together through terephthalic acid and was prepared with hydrothermal method[1]. Resulted Nanorods coating is as sorbent phase on the fused silica fiber as novel solid phase microextraction. The SEM images with scanning electron microscopy conforms the formation nanorods (100-200 nm diameter) with high surface area[2]. The headspace solid phase microextraction (HS-SPME) method was applied for pre-concentration and analysis some cancer biomarkers included 1,2,4-trimethyl benzene, phenol, 2-pentanon from urine samples for testing the selectivity of the prepared fiber[3]. The variables affected on the extraction efficiency were optimized by experimental design method. The variables are extraction temperature and time, pH, salt% (w/v) and desorption time [4]. The calibration curves were obtained in the concentration range from 10-350 ppb for all targets. The limits of detection and limit of quantitation values were achieved 1 μg l-1 and 10 μg l-1 for 1,2,4-trimethyl benzene same as 2-pentanon and 5 μg l-1 and 50 μg l-1 for phenol, respectively. The relative standard deviations was calculated for one fiber and fiber to fiber in the range 3-15%. Finally the results obtained suggested Fe3O[C6H4(CO2)2]3.nH2O SPME fiber can be used for extraction of biomarkers from urine samples in the use of analysis cancer diagnostic and environmental compounds.