Neurotoxic Effects of Stanozolol on Male Rats’ Hippocampi: Does it Cause Apoptosis

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 410

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شناسه ملی سند علمی:

NIMED03_211

تاریخ نمایه سازی: 7 آبان 1398

چکیده مقاله:

Stanozolol is the most appealing drug of Anabolic-Androgenic Steroids that is abused by athletes in high doses for improving their energy, appearance and physical size. Being proved before, Stanozolol causes changes in behaviour and has various physical effects.Studies have been conducted on its neurotoxic impacts on CNS that are mostly psychological-based. Current study was performed to investigate the apoptotic effect of Stanozolol on different parts of rat’s hippocampus. Materials and Methods: Sixteen male Wistar rats weredivided randomly and equally into two groups (control and experimental). The experimental group received subcutaneous injections of Stanozolol (5mg/Kg/day) for 28 days uninterruptedly. The control group was treated with normal saline in the same manner. After routineprocedures, the coronal sections of rats’ brains were stained with Toluidine blue and TUNEL for pre-apoptotic and apoptotic cell detection respectively. In order to compare the groups, the mean number of TUNELpositive and pre-apoptotic neurons per unit area were calculated and analyzed. Results: Our histopathological examination revealed the mean number of pre-apoptotic and apoptotic neurons in the CA1, CA2, CA3 and DG area of hippocampus have significantly increased in Stanozolol may induce pre-apoptotic and apoptotic cell formation in different regions of hippocampus.

نویسندگان

Faezeh Nemati Karimooy

Binaloud Healthcare Network, Mashhad University of Medical Sciences, Mashhad, Iran

Alireza Ebrahimzadeh Bideskan

Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abbas Mohammadi Pour

Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Seyed Mahmoud Hoseini

Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran