Effects of Platelet-Rich Plasma (PRP) on the B92 Cell Primed with Killed Preparation of Escherichia Coli

Some evidence indicated that Platelet- Rich Plasma (PRP) possesses anti-inflammatory and regenerative benefits. The purpose of the present study was to investigate the protective effects of PRP on the rat Glial/neuronal cell line B92 after exposure with killed preparation of Escherichia Coli. Materials and Methods: B92 cells were cultured in the absence or presence of E. coli (1:100 ratio) for 24 h in DMEMmedium supplemented with FBS and different concentration of PRP (0,5,10 and 20%).Cellular cytotoxicity was measured by MTT assay. Results: Killed preparation of E. Coli reduced the proliferation of B92 cells in a concentration- dependent manner. PRP at 10 or 20 % concentrations could reverse this reduction so that in the presence of 20% PRP, the proliferation of B92 cells were more profound that un-pulsed B92 with E.coli. Nevertheless, PRP at 5% didn’t show any significant effects on the proliferation of B92 cells primed with E. coli. Conclusion: In vitro data showed that PRP has protective effects against cell death induced by E. coli in Glial/neuronal cell line B92