Effect of 5-HT1D Receptor Agonist on Hippocampal Long-Term Potentiation and Level of BDNF in Reinstated-Methamphetamine Rats
سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 460
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شناسه ملی سند علمی:
NIMED03_165
تاریخ نمایه سازی: 7 آبان 1398
چکیده مقاله:
Methamphetamine (METH) is a psychostimulant that the precise mechanisms of its effects remain unknown and current relapsetreatments have low efficacy. However, brain-derived neurotrophic factor (BDNF) and neuronal plasticity are essential contributors, despite paradoxical reports and a lack of comprehensive studies. Serotonin is a key neuromodulator in this challenge. While there isa known distribution of 5-HT1D receptor in the reward and memory areas, the physiological function of this receptor is currently unknown. Here, we evaluated effect of a 5-HT1D receptor agonist (PNU142633) on the electrophysiology recording and level of BDNF.Materials and Methods: Rats were implanted with a cannula into lateral ventricle. After recovery, animals treated with saline or METH (5 mg/kg) during acquisition of conditioned place preference (CPP). On day 13 extinction, METH groups divided into four groups.They received METH (0: saline, 1, or 2.5 mg/kg; i.p.) +vehicle (5 μl/rat) or a priming dose of METH + PNU (2 μg/5 μL; i.c.v.) and conditioning scores were calculated on reinstatement day. Finally, electrophysiology and western blotting tests were performed for measurementsLTP components and expression of BDNF, respectively. Results: The results showed pre-treatment with PNU significantly reduced the reinstatement of priming METH seeking and level of BDNF expression was more than other groups. Furthermore, PS amplitude andEPSP slope in vehicle +priming METH rats were more than others. Also, PS amplitude in PNU+METH group was lower than priming METH group. Conclusions: These findings suggest that PNU can alter synaptic transmission and level of BDNF. Subsequently havetherapeutic potential for preventing METH relapse
نویسندگان
Reihaneh Sadeghian
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Siamak Shahidi
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Alireza Komaki
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Sara Soleimani Asl
Department of Anatomy, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran