Zinc Oxide Nanoparticles Synthesized by the Green Method from the Extract of the Hyssopus Officinalis Plant and Their Anti-Cancer Effect on Brain Cells and Effect on the Toxicity of These Nanoparticles on Brain Tissue: In Vivo and in Vitro Studies

The explicit bio-safety issue and potential hazard of nanoparticles (NPs) on various organelles can be considered as a major challenge.Hence, there is a need to appraise the safety problems of these NPs. In the present study, we have determined the green synthesis of ZnO nanoparticles using Hyssop (Hyssopus officinalis) extract and their effects on U87 cell line and BALB/c mice. Materials and Methods:The cytotoxicity of the ZnO NPs was assessed on U87 cell line by MTT test after characterization with various analytical techniques. The weight of brain tissue and histopathological assessments carried out as in vivo analysis in BALB/c adult male mice. Results:The obtained IC50 for U87 treated with ZnO NPs for 24 and 48 hours Respectively was 35 μg/ml and 23 μg/ ml. From the aspect of histopathological, they did not change, but these nanoparticles slightly increased the weight of the brain tissue. All expenses of this researchwere done personally. Conclusion: The results of the analysis of MTT assay for u87 cell lines showed significant IC50 values, with increasing concentrations and increasing time, had a greater fecundity effect on the cells. The results obtained in this work contribute to increase the knowledge on the genotoxic and cytotoxic potential of ZnO NPs in general, and specifically on human neuronal cells, but further investigations are required to understand the action mechanism underlying the cytotoxic and genotoxic effects observed.