Effect of Levodopa and Benserazide and Combinational Therapy on Histopathological Changes in SNC and Hippocampus and AlsoOxidative Stress Enzymes in Serum of the Parkinsonian Rats

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 411

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شناسه ملی سند علمی:

NIMED03_065

تاریخ نمایه سازی: 7 آبان 1398

چکیده مقاله:

Parkinson’s disease is a progressive and chronic disorder of the central nervous system.Levodopa and dopaminergic agonists are not used alone, but also combined with other drugs such as benserazide. The aim of this study was to investigate the effects of levodopa and benserazide on histopathological changes in the substantia nigra, and the changes of superoxide dismutase and glutathione peroxidase enzymes in serum of the Parkinsonian rats. Materials and Methods: Twenty male Wistar rats were studied in this project.Mouses were divided into five groups: control, sham, levodopa, benserazide, levodopa and benserazide.Levodopa was administered at a dose of 10 mg / kg, benserazide at a dose of 20 mg / kg, and in the combined group, 30 mg / kg intraperitoneally (ip) were injected into the mouses. Results: The overall comparison of the five groups showed a significant difference between the groups for the level of SOD enzyme (p = 0.002). The post hoc test showed a significant difference between the combination group of levodopa and benserazide with levodopa (p = 0.035) and benserazide (p = 0.001) groups, so that the SOD level in the combinationgroup was 10.73 ± 3.1 and 15.93 ± 3.1 respectively, which was more than the previous two groups, but the level of the GPX enzyme did not show significant differences between the groups (p = 0.084). About the histopathological changes, counting the number of cells showed a significant difference between the combinational group (levodopa+benserazide) compared to the MPTP group. Conclusion: According to theresults, increasing SOD levels will neutralize oxidative stress factors and free radicals, which will improve the cell’s efficiency and survive.

نویسندگان

Hamed Farzadmanesh

Nervous System Stem Cells Research Center, Department of Anatomy, Semnan University of Medical Sciences, Semnan, Iran

Mohammad Reza Madadian

Faculty of Medicine, Branch of North Tehran, Islamic Azad University, Tehran, Iran

Marzyeh Salami

Department of Biochemistry, Semnan University of Medical Sciences, Semnan, Iran

Ali Ghanbari

Department of Physiology, Semnan University of Medical Sciences, Semnan, Iran