The Effect of L-Carnitine on The Uterine Tubes and Uterus Histology in The Mouse Model of The Endometriosis

Background: Endometriosis is a female condition that causes complications in the reproductive system, especially in the uter-ine and uterine tubes, and has a direct relationship with infertil-ity. So improving endometriosis improves fertility. L-carnitine, based on improved metabolism and antioxidant role, can be a solution to improve the complications of endometriosis on the uterus and uterine tubes and improve their histology. The target of this study was to investigate the effects of L-carnitine on uterus and uterine tubes histology in mouse model of endome-triosis.Materials and Methods: At the first stage, induction of endo-metriosis in mice was done by injection of suspension of endo-metrium separated from the uterus of donor mice. In the sec-ond stage, the control group was divided into four treatments: control without any treatment, control receiving L-carnitine intrapritonally (250 mg/1kg, every day), control receiving da-nazol intrapritoneally (25 mg/1kg, every other day) and control receiving concurrently L- carnitine and danazol. The endome-triosis group was divided into four treatments: endometriosis without any treatment, endometriosis receiving L- carnitine intrapritoneally (250 mg/1kg, every day), endometriosis receiv-ing danazol intrapritoneally (25 mg/1kg, every other day), and endometriosis receiving concurrently L-carnitine and danazol. At the end of the treatment period, the mice were dissected then uterus and uterine tubes were separated and histologically ex-amined.Results: The results showed that inducing endometriosis cuss-ed serious problems in uterus and uterine tubes such as severe inflammation, fibrosis, increasing proliferation and secretion of epithelial cells, dilating of blood vessels, decreasing cilia in uterine tubes, increasing of foamy discharge in the lumen of uterine tubes and the closure of the channels. Microscopic ex-amination showed that l-carnitine improves some of the compli-cations of endometriosis, such as decreasing cell proliferation of endometrial cells and their secretion, reducing inflammation and fibrosis. Also Injection of danazol improved of endometrio-sis, such as reducing cell proliferation, decreasing secretions and adhesions in uterine tubes and the channels were almost open. Simultaneous administration of L-carnitine and danazol more reduced the complications of endometriosis, and the ani-mal s estrus cycle was more regularized.Conclusion: Since endometriosis has a bad effects on the uter-us and uterine tubes and this has a direct relationship with in-fertility, we can increase the fertility rate by reducing the harm-ful effects of endometriosis on the uterus and uterine tubes. Therefore, it can be concluded that treatment with danazol and L-carnitine improving the condition of the uterus and uterine tubes compared to the without any treatment

کلیدواژه ها:

Endometriosis ، L-Carnitine ، Uterus ، Uterine Tubes ، Mouse