The Effect of Sperm DNA Fragmentation on Param-eters of Sperm, Blastocyst Formation, Embryo Quality and Pragnance Rate in Assisted Reproductive Technique(ICSI)

Background: The aim of the present study was to investigate the correlation between sperm DNA fragmentation standard laboratory and parameters of human semen. We have also ex-amined an impact of sperm DNA damage on embryo quality and Blastocyst Formation after ICSI procedures.Materials and Methods: Semen samples from 50 men have been analyzed as a part of diagnostic semen analysis and fertil-ity evaluation by using conventional microscopic semen analy-ses. In each sample, the concentration, motility, morphology and vitality of spermatozoa were evaluated. Sperm DNA dam-age was determined by using Halo sperm test with threshold value of DNA fragmentation index (DFI) at (<15%, 15-20% ,> 30 %). Embryo quality was evaluated undergoing assisted re-production technique (ART) procedures at our clinic.Results: The results of this study indicate that the patient group with DFI ≥ 30 % had significantly lower values of standard se-men parameters, Blastocyst Formation and embryo quality than the group of patients with DFI index(<15%,15-20%). In addi-tion Negative correlations were found between DNA fragmen-tation and motility (r = -0.41, p < 0.001), morphology (r = -0.32, p = 0.001) and vitality (r = -0.36, p < 0.001). We also found that in the group of patients with DFI index (<15%,15-20%) the were significantly more embryos of better quality.Conclusion: In men with poorer semen quality, evaluated by standard semen parameters, a higher proportion of sperm with damaged DNA can also be expected. Higher sperm DNA dam-age, established by Halos perm test, also had an impact on em-bryo quality in this group of patients.

کلیدواژه ها:

DNA Fragmentation ، Parameters Sperm ، Blastocyst Formation