Extended Incubation of Mouse Spermatozoa Affects the Preimplantation Embryonic Development

Background: Sperm quality is a determinant factor on the out-come of assisted reproduction and is influenced by many fac-tors, such as time and temperature during in-vitro incubation of sperm. This study investigated the effects of long-term in vitro sperm incubation at room temperature (RT) on Sperm quality parameters and early embryonic development.Materials and Methods: Mouse sperm samples were divided in to 2 groups (fresh and incubated at RT for 24 hours). sperm parameters were assessed according to WHO guidelines. DNA Fragmentation index (%DFI) was evaluated by SCSA. Mouse sperm were microinjected into mouse oocytes. Fertilization rete and embryo development were evaluated.Results: The percentage of progressive motility signifi - cantly decreased in samples incubated at RT compared with fresh group (P <0.05). The percentage of DFI was not significantly altered in samples incubated at RT compared with fresh group (P> 0.05). The morula and blastocyst rate significantly decreased after 24 hours incubation at RT (P <0.05).Conclusion: The result of this study demonstrated that extend-ed sperm incubation affects preimplantation embryonic devel-opment.

کلیدواژه ها:

Sperm Incubation ، Embryo Development ، DNA Fragmentation