Improvement of In Vitro Sperm Movement Charac-teristics in Normozoospermic and Asthenozoospermic Men by Repaglinide

Background: Human sperm activity must be precisely regu-lated to achieve natural fertilization. The human sperm motil-ity and hyperactivation induce by the different factors such as intracellular calcium concentration. Repaglinide is one of the current antidiabetic drugs that decrease blood glucose level by inducing the release of insulin in pancreatic islets. Mechanismaction of repaglinide is blocked of ATP-sensitive potassium channels and depolarization of b- cell membrane, opening the voltage-gated calcium channels and then increasing in intracel-lular calcium. In this study; we examined the effect of repa-glinide on in vitro induction of the human sperm motility and hyperactivated motility.Materials and Methods: Semen samples were collected from two groups of normozoospermic donors and asthenozoosper-mic patients that were washed free of seminal plasma and then treated with medium alone (control) and were treated with 100 nM and 1µM concentration of repaglinide. After 1 hour of incu-bation for all treatments, the percentage of sperm motility and sperm hyperactivation were assessed.Results: Results showed that repaglinide at concentration of 100 nM and 1µM caused significantly improvement of in vitro induction of sperm motility and hyperactivated motility in both groups, but in normozoospermic 1µM concentration of repa-glinde has obviously effect on progressive motility and in asthe-nozoospermic group, highest hyperactivated motility rate was seen in 100 nM concentration of repaglinide in comparison to 1µM concentration and control (p < 0.05).Conclusion: In conclusion our results suggest that repaglinide can improve sperm motility and hyperactivity in normozoo-spermic and asthenozoospermic men.

کلیدواژه ها:

Repaglinide ، Motility ، Sperm ، Hyperactivation