Effect of Myoinositol Supplement on The Quality of Frozen- Thawed Human Sperm on Patients with Oligoas-thenoteratozoospermia Syndrome

Background: Oligoasthenoteratozoospermic syndrome is com-mon presentation in male infertility. this syndrome occurs by decreasing motility, numbers and morphologic changes sperm. The production of oxygen species increases, in the semen ab-normal. then it may affect motility, morphology and DNA sta-bility of sperm. This study aimed at evaluating the effect of My-oinositol in human semen parameters and DNA fragmentation patients with syndrome after thawing procedure.Materials and Methods: Semen samples were obtained from 40 oligoasthenotetratozoospermia patients, aged 28-45, which have been referred to IVF center of Akbar Abadi hospital. Se-men samples were collected through masturbation into sterile containers, following 2–4 days sexual abstinence. All the sam-ples after by CASA were immediately in two groups, group 1: (2 mg per ml) + freezing medium (Life Global), group 2:freezing medium. Samples were thawed. Semen parameters in two groups were analyzed by CASA for motility and morphol-ogy. Also, the level of total ROS, TAC, DNA fragmentation and MDA were evaluated by DCFHby Fluorimetry), ELISA kit, TUNEL assay by flow cytometry and ELISA respectively.Results: Our data clearly showed that total, progressive motil-ity were higher in the experimental group. Also, DNA fragmen-tation is lower in myoinositol than control(22.4% vs. 29.6%, p0> 001). TAC is higher in myoinositol than control (1.12mM vs. 0.95 mM, P> 0.05) and MDA is significantly decreased in myoinositol than control (2.1 nmol vs. 2.3 nmol, P<0.05). The level of ROS was decreased in myoinositol group but not sig-nificant (179.4mM vs. 200.8mM, P<0.05).Conclusion: These data suggest that improves sperm param-eters through the mitochondrial mechanism and it can play a protective role sperm DNA and increases TAC capacity. So, This study showed that can be used as a supplement in sperm freezing process in patients with Oligoasthenoteratozoospermia syndrome.

کلیدواژه ها:

Myoinositol ، Sperm Cryopreservation ، DNA Dam-age ، Oligoasthenoteratozoospermi Syndrome