Background: The human fallopian tube is a part of female reproductive system in which fertilization occurs. The cross-talk between sperms and fallopian tube epithelial cells is very valuable because it has different roles in reproductive process such as; fertilization and early embryo development. Therefore, the existence of a sperm recognition system that apprise the fe-male reproductive tracts of the sperm entry looks essential. This system should prepare the safe environment in fallopian tubes in which sperms and oocytes transport and eventually fertili-zation occur. It has been suggested, some kind of chemokines can regulate the ability of female reproductive tract to tolerate the allogenic spermatozoa. Therefore, in this study we evalu-ate influence of the DNA-fragmented sperms on fallopian tube epithelial cells by assessing chemokines expression with PCR-array and also we investigate association between sperm DNA Fragmentation Index (DFI) and pregnancy rate outcome.Materials and Methods: In this investigation, sperm samples from 20 donors with normal features according to WHO stand-ards were collected. Based on DNA fragmentation we evalu-ated normal and abnormal sperm with tunnel assay method. To assess the effect of spermatozoa on human fallopian tube epi-thelial cells, sperms were co-cultured with fallopian tube cell line (OE-E6/E7). After 24 hours’ cells were washed and RNA extraction was performed. Finally, cDNA was synthetized and PCR array was performed. In the present study we performed statistical analysis of CCL21, CCL22, CXCL2 and CXCL5.Results: The outcome of data analysis shows that DNA frag-mentation of sperms can change the expression of chemokines relatively. The highest expression of CCL21 and CCL22 were seen in the presence of normal sperms. However, there was no significant difference between abnormal DFI and the con-trol groups. On the other hand, the expression of CXCL2 and CXCL5 were the lowest under the effect of sperms with DNA fragmentation.Conclusion: The results show that fallopian tube response to the presence of sperms with different mechanism including chemokine alteration which might lead to modulate the fallo-pian tubes environment for fertilization. Different expression of chemokines in normal and abnormal DFI groups also shows that the necessity of DFI screening for ART procedure.