Evaluating the Potential of Dj-1 and Sap as Two Bio-markers for Human Sperm Selection

Background: The selection of sperm with good genomic in-tegrity and surface antigens is a critical factor in the success of assisted reproductive technology (ART) especially in male fac-tor infertility. This study assessed the potential DJ-1 and serum amyloid P compound (SAP) two sperm surface pro teomes as bio markers for sperm selection.Materials and Methods: Sperm were obtained from 114 men (72 asthenoteratozoospermic (AT) and 42 normospermic(N) who presented at Avicenna Fertility Clinic for their treatment. The semen characteristic, DNA fragmentation (DFI), chromatin maturation index ( CMI ), bio marker levels on sperm, and their correlation with embryo quality were assessed in both groups. The paired samples t-test and independent-samples t-test were used for analyzing the data and P values<0.05 were considered significant.Results: Outcomes exhibited the major reduction in DJ-1 and SAP following reduction in sperm quality and DNA integrity (P<0.001) with cut-off value of 12% (DJ-1) and 10% (SAP). The specificity of these three biomarkers was 73.8 and 88.1%, respectively. Also, DFI (P<0.001), CMI (P<0.05), cleavage (P<0.05), and embryos quality (P<0.001) decreased signifi-cantly in AT group in compared with NS group. It was shown that DFI was 76.5% in DJ-1 and 94.1% in SAP, and CMI was 75.50% and 87.5%, respectively. The significant correlation was found between of the two bio markers and CMI (P<0.001), DFI (P<0.001) and embryos quality (P<0.001).Conclusion: By comparing the efficiency of these two bio markers for selecting sperm with the lowest level of chromatin damages, it seems that selection based on SAP has significance over others.

کلیدواژه ها:

Serum Amyloid P Component ، DJ-1 ، Chromatin Maturation ، Sperm DNA