No Titletherapeutic Properties of Mesenchymal Stem Cell Secretom or Silybum Marianum Extract on Ccl4- In-duced Liver Failure Mice

Background: Due to some limitation in term of liver disease treatments including restriction to adequate donated liver and complication of liver transplantation, applying of new curative approaches are more valuable. Recently, mesenchymal stem cell (MSCs) and their derivatives including macrovesicle, har-vested conditioned medium and exosome are used to treat dif-ferent sever diseases. MSC conditioned medium contains sev-eral cytokines, growth factors and other bio-reactive molecules. On the other hand, Herbal extract is usually in term of disease treatment. In this regard we conducted this study to compare the herbal Silybum marianum extract to MSC-conditioned medium (MSC-CdM) therapeutic potentialities as a potential novel strategy in term of liver failure treatment.Materials and Methods: Silybum marianum extract was purchased and human amniotic membrane-derived MSC-con-ditioned medium was harvested and sterilized using 0.22 μm filters. CCl4 induced-liver failure mice models (LF) was estab-lished and confirmed by biochemical and histological analysis according to our previous study. 4 animal groups containing 10 mice, were designed as follows, normal mice receiving phos-phate buffer solution (PBS) as normal control, LF-receiving PBS as sham, LF- receiving Silybum marianum extract as LF-Sm and LF- receiving MSC-CdM as LF-CdM. All treat-ments were done 24 hours post CCL4 injection. Biochemical, histopathological and 14-days-survival rate assay was applied to evaluate hepatoprotective properties of Silybum marianum extract and MSC-CdM in different interval times, 2, 4, 7, 10, 14 days after administration of Silybum marianum extract and MSC-CdM.Results: According to biochemical assay 2 and 4 days post-treatment, alanine aminotransferase (ALT) Aspartate transami-nase (AST) were significantly increased in the sham group comparing to the normal group, however, these mentioned en-zyme values were significantly lower in the LF- Sm, and LF-CdM groups compared to sham (P<0.05). The histopathological assay showed a dramatically decreased number of inflamma-tory cells and presence of repaired cells in LF- CdM mice. LF-Sm mice revealed mild necrosis and inflammation compared to bridging necrosis in sham. Next, comparing LF- Sm group to LF-CdM group, significantly lower values of AST was detected 4 days after injection in LF-CdM group. However, these two groups showed no significant difference in liver enzyme levels 2 days after injection. LF-CdM showed rapid liver control and less mortality rate compared to other experimental groups.Conclusion: Both Silybum marianum extract and MSC-condi-tioned medium have demonstrated hepatoprotective properties, however less mortality rate in LF-CdM showing a promising future an adjuvant therapy for liver disease.

کلیدواژه ها:

Mesenchymal Stem Cell ، Conditioned Medium ، Sily-bum Marianum ، Liver Failure