Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 22، شماره: 2
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 373
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شناسه ملی سند علمی:
JR_IJBMS-22-2_003
تاریخ نمایه سازی: 20 مهر 1398
چکیده مقاله:
Objective(s): It is known that extremely low frequency-pulsed electromagnetic fields (ELF-PEMF) influence multiple cellular and molecular processes. Retinal pigment epithelial (RPE) cells have a significant part in the emergence and pathophysiology of several ocular disorders, such as neovascularization. This study assessed the impact of ELF-PEMF on the proangiogenic features of RPE cells. Materials and Methods: Primary cultured RPE cells were treated with ELF-PEMF (50 Hz) for three days. Using ELISA assay, we evaluated the effects of treatment on RPE cell proliferation and apoptosis. Also, RT-PCR was used to determine the gene expression of proangiogenic factors, such as matrix metalloproteinase-2 (MMP-2), MMP-9, vascular endothelial growth factors receptor 2 (VEGFR-2), hypoxia-inducible factor 1 (HIF-1α), VEGFA, cathepsin D, connective tissue growth factor (CTGF), E2F3, tissue inhibitors of metalloproteinases 1 (TIMP-1), and TIMP-2.Results: No noticeable changes were observed in cell proliferation and cell death of ELF-PEMF-exposed RPE cells, while transcript levels of proangiogenic genes (HIF-1α, VEGFA, VEGFR-2, CTGF, cathepsin D, TIMP-1, E2F3, MMP-2, and MMP-9) increased significantly.Conclusion: RPE cells are important for homeostasis of the retina. ELF-PEMF increased the gene expression of proangiogenic factors in RPE cells, which highlights concerns about the impact of this treatment on human health.
کلیدواژه ها:
نویسندگان
Morteza Oladnabi
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran|Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abouzar Bagheri
Department of Clinical Biochemistry and Genetics, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Mozhgan Rezaei Kanavi
Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abbas Azadmehr
Immunology Department, Babol University of Medical Sciences, Babol, Iran
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